Question

Label each of the four mutated DNA segments (a-d) below according to the type of mutation each represents. Use the codon chart to determine how each mutation would impact the amino acid for each segment. Wild-type DNA DNA template strand 3' TACGCAGGTACCATC5 S' ATGCGTCCATGCTAG 3' MANA 5 AUGCGUCCAUGCUAC 3 protein MA Pret - instead of DA 3* TAccccccTACCATc s' ONA 3' TACGCAGGTACTATC 5 5' ATGCGGCCATGCTAG 3 5' ATGCGTCCATGATAG 3 mRNA 5' AUGCGGCCAUGCUAG 3 mRNAS AUGCGUCCAUGAUAG 3' ol -Extrac A instead of G DNA 3' TACGCAAGTACGATC 5 S' ATGCGTTCATGCTAG 3' mRNA 5' AUGCCUUCAUGCUAG 3 ONA 3 TACCCCAGCTACGATC 5 SATGGCGTCCATGCTAG 3 mRNAS' AUCGCGUCCAUGCUAG 3' [Choose [Choose nonsense frameshift base analog silent tautomer missense [Choose)

Answer 1

ORIGINAL PROTIEN SEQUENCE

AUG CGU CCA UGC UAG.

MET- ARG-PRO-CYS-STOP

Answer A : SILENT MUTATION

mRNA sequence given after mutation is

AUG CGG CCA UGC UAG.

MET- ARG- PRO -CYS-STOP

In above case the mutation is SILENT mutation, as the mutation has caused no observable effect on the protein sequence.

Answer B : NON SENSE MUTATION

mRNA sequence given after mutation is

AUG CGU CAA UGA UAG

MET- ARG-GLY-STOP-STOP

In above case the mutation is NON SENSE mutation, as the mutation has caused a sense codon to change in non sense codon that is it has changed to chain –termination codon.

Answer C : MIS SENSE MUTATION

mRNA sequence given after mutation is

AUG CGU UCA UGC UAG

MET- ARG-SER-CYS-STOP

In above case the mutation is MIS SENSE MUTATION, as it is point mutation which has lead to coding of different amino acid in the protein sequence.

Answer D : FRAME SHIFT MUTATION

mRNA sequence given after mutation is

AUG GCG UCC AUG CUA G

MET- ALA-SER-MET-LEU

In above case the mutation is frame shift mutation, as it is caused by addition of a base pair in the DNA of the gene which has lead to translation of genetic code in an unnatural reading frame from the point of addition of base pair to end of gene