You mix 3 solutions: 0.5 L of 10 mM Tris-HCl 1.5 mL of 1 M Tris-HCl...
You mix 3 solutions: 0.5 L of 10 mM Tris-HCl 1.5 mL of 1 M Tris-HCl 200 mL of NaCl at 1.2%(w/v) (MW: 58.44) What is the final concentration of NaCl, in mM?
Homework Answers
1L=1000 mL
0.5 L= 0.5
1000=500
mL
final volume= 500+200+1.5=701.5 mL
701.5 mL=701.5/1000=0.7015 L
for NaCl
M1=1.2%
M2=?
V1=200 mL
V2= 701.5 mL
M1V1=M2V2
M2= M1V1/V2
= (1.2200)/701.5
=0.342 %
(mass/volume)100=0.342%
(mass/701.5)=0.342%/100
mass= (0.342/100)701.5
= 2.4 g
number of moles= mass in gram/gram molecular mass
= 2.4/58.44
= 0.041
molarity= number of moles/volume of the solution in litres
= 0.041/0.7015
= 0.0585 M
1 M= 1000 mM
so the concentration of NaCl in mM=58.5 mM
Add Answer to:
You mix 3 solutions: 0.5 L of 10 mM Tris-HCl 1.5 mL of 1 M
Tris-HCl...
are making 1 L of Ni-NTA Buffer (100 mM Tris, 200 mm NaCl, pH 7.8). a)...
are making 1 L of Ni-NTA Buffer (100 mM Tris, 200 mm NaCl, pH 7.8). a) Calculate the mass of NaCl (58.44 MW) to add to the buffer. b) Calculate the mass of Tris base (121.1 MW) to add to the buffer. c) You then measure the pH of the buffer and find it to be 8.2. What amount of 1 M HCl must be added to reach a pH of 7.8? pKa of Tris is 8.06. Ignore volume change...
Making Solutions Practice Problems: I. How would you prepare 100 ml of a 30% (w/v) solution...
Making Solutions Practice Problems: I. How would you prepare 100 ml of a 30% (w/v) solution of polyethylene glycol (PEG)? 2. How would you prepare 50 ml of a 9 % (w /v ) solution of NaCI? 3. How would you prepare 200 ml of 70 % ( v /v ) solution of ethanol from a stock of 95% ethanol? How would you prepare 200 ml of 0.3 M NaCl? (NaCl MW 58.44) 4. 5. How are 50 ml of...
What is pH of a solution if you mix: 10 ml of 0.2 M HCl and...
What is pH of a solution if you mix: 10 ml of 0.2 M HCl and 35 ml of 0.15 M NaOH. 200 ml of 20 mM NaOH and 100 ml of 40 mM HCl. 200 ml of 250mu M NaOH and 25 ml of400 mu M H_2 SO_4. How many grams NaCl is is needed to prepare 600 mL of 250 ppm Na solution?
4. A buffer you want to use for protein purification calls for the following components: Tris-HCI...
4. A buffer you want to use for protein purification calls for the following components: Tris-HCI (110 mM, MW = 121.14 g/mol) Urea (0.075 M, 60.06 g/mol) NaClI (150 mM, FW 58.44 g/mol) Dithiothreitol (4 mM, MW = 154.25 g/mol) B-Mercaptoethanol (50 mM, MW 78.13 g/mol) You need to make 2500 mL of this buffer. In your lab you have a bottle of 500 mM Tris-HCl, 125 mM β-mercaptoethanol, and solid urea, NaCl, and dithiothreitol. Calculate the amount of each...
Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock...
please hlep me answer those three questions asap.
Please ignore question 7.
Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock solutions from which you can make the buffer; 0.6 M NaH2PO4 (the acid form) and 0.6 M Na2HP04 (the base form). You need to add 3 times more of the base form than the acid form to achieve the desired pH. How will you make this solution? Question 8...
3. A common buffer used in working with DNA solutions is tris. tris a) Draw the...
3. A common buffer used in working with DNA solutions is tris. tris a) Draw the structure of the protonated form of tris. HO Но, b) When 30.00 mL of a 62.20 mM tris solution is titrated with 0.100 M HCl, the pH equals 7.40 after 3.96 mL of HCl are added. What is the PK of tris? c) (Optional, not counted) How would you prepare 500.0 mL of a 0.0100 M PH 7.4 tris buffer solution assuming you had...
1. in run 1, you mix 8.1 mL of the 43 g/L MO solution (MO molar...
1. in run 1, you mix 8.1 mL of the 43 g/L MO solution (MO molar mass is 327.33 g/mol), 2.89 mL of the 0.040 M SnCl22 in 2 M HCl solution, 5.46 mL of 2.0M HCl solution, and 3.47 mL of 2.0M NaCl solution. What is the [MO]? a. In run 1, you mix 8.1 mL of the 43 g/L MO solution (MO molar mass is 327.33 g/mol), 2.89 mL of the 0.040 M SnCl22 in 2.0 M HCl...
1.Show calculations for making following reagents A. 200 ml of a 1M solution of Tris (molecular...
1.Show calculations for making following reagents A. 200 ml of a 1M solution of Tris (molecular weight 121.1 g/mol) B. 300 ml of 0.2M EDTA (MW 372.2 g/mol) C. 200 ML of TE buffer (contains 10 mM Tris and 1 mm EDTA) using solutions made in A and B A solution containing a concentration of 0.01M (H+) ions will have a pH of --- 3. What is the Henderson Hasselbach equation? Use this equation to...
please check my answer if i did right Making solutions with multiple solutes (Part II) 0.15...
please check my answer if i did right
Making solutions with multiple solutes (Part II) 0.15 molo / 17.582 9 gnad 1. (5 points) Describe how to prepare a 2L solution: 15 mol 250 mM NaCl 58.449/md Y2LX 0.15 m 1% NP-40 (v/ve) 0.01 X 26 2 9 0.1% SDS (w/v), 0.001% 2L 0.0029 / SAS 50 mM Tris-HCI 0.05 X 62.14 = 6.0579 16.057 g o Hell + DI Hgo to a I to o to 2. (4 points)...
Solutions practice problems. calculate how much of each ingredient you would need to make the solution...
Solutions practice problems. calculate how much of each ingredient you would need to make the solution CIRCLE YOUR FINAL ANSWERS. Be sure to include the correct units with your answer. Molecular weights: SDS-288.38 g/mol Tris (base) = 121.1 g/mol EDTA = 372.2 g/mol Sodium acetate 82.03 g/mol Tris maleate = 237.2 g/mol Magnesium sulfate heptahyrdate=246.5 g/mol Sodium hydroxide - 40.00 g/mol (can calculate the MW for simple compounds) 1) 200 mL of 25% SOS 2) 60 ml alkaline lysis buffer...
are making 1 L of Ni-NTA Buffer (100 mM Tris, 200 mm NaCl, pH 7.8). a) Calculate the mass of NaCl (58.44 MW) to add to the buffer. b) Calculate the mass of Tris base (121.1 MW) to add to the buffer. c) You then measure the pH of the buffer and find it to be 8.2. What amount of 1 M HCl must be added to reach a pH of 7.8? pKa of Tris is 8.06. Ignore volume change...
Making Solutions Practice Problems: I. How would you prepare 100 ml of a 30% (w/v) solution of polyethylene glycol (PEG)? 2. How would you prepare 50 ml of a 9 % (w /v ) solution of NaCI? 3. How would you prepare 200 ml of 70 % ( v /v ) solution of ethanol from a stock of 95% ethanol? How would you prepare 200 ml of 0.3 M NaCl? (NaCl MW 58.44) 4. 5. How are 50 ml of...
What is pH of a solution if you mix: 10 ml of 0.2 M HCl and 35 ml of 0.15 M NaOH. 200 ml of 20 mM NaOH and 100 ml of 40 mM HCl. 200 ml of 250mu M NaOH and 25 ml of400 mu M H_2 SO_4. How many grams NaCl is is needed to prepare 600 mL of 250 ppm Na solution?
4. A buffer you want to use for protein purification calls for the following components: Tris-HCI (110 mM, MW = 121.14 g/mol) Urea (0.075 M, 60.06 g/mol) NaClI (150 mM, FW 58.44 g/mol) Dithiothreitol (4 mM, MW = 154.25 g/mol) B-Mercaptoethanol (50 mM, MW 78.13 g/mol) You need to make 2500 mL of this buffer. In your lab you have a bottle of 500 mM Tris-HCl, 125 mM β-mercaptoethanol, and solid urea, NaCl, and dithiothreitol. Calculate the amount of each...
please hlep me answer those three questions asap.
Please ignore question 7.
Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock solutions from which you can make the buffer; 0.6 M NaH2PO4 (the acid form) and 0.6 M Na2HP04 (the base form). You need to add 3 times more of the base form than the acid form to achieve the desired pH. How will you make this solution? Question 8...
3. A common buffer used in working with DNA solutions is tris. tris a) Draw the structure of the protonated form of tris. HO Но, b) When 30.00 mL of a 62.20 mM tris solution is titrated with 0.100 M HCl, the pH equals 7.40 after 3.96 mL of HCl are added. What is the PK of tris? c) (Optional, not counted) How would you prepare 500.0 mL of a 0.0100 M PH 7.4 tris buffer solution assuming you had...
please check my answer if i did right
Making solutions with multiple solutes (Part II) 0.15 molo / 17.582 9 gnad 1. (5 points) Describe how to prepare a 2L solution: 15 mol 250 mM NaCl 58.449/md Y2LX 0.15 m 1% NP-40 (v/ve) 0.01 X 26 2 9 0.1% SDS (w/v), 0.001% 2L 0.0029 / SAS 50 mM Tris-HCI 0.05 X 62.14 = 6.0579 16.057 g o Hell + DI Hgo to a I to o to 2. (4 points)...
Solutions practice problems. calculate how much of each ingredient you would need to make the solution CIRCLE YOUR FINAL ANSWERS. Be sure to include the correct units with your answer. Molecular weights: SDS-288.38 g/mol Tris (base) = 121.1 g/mol EDTA = 372.2 g/mol Sodium acetate 82.03 g/mol Tris maleate = 237.2 g/mol Magnesium sulfate heptahyrdate=246.5 g/mol Sodium hydroxide - 40.00 g/mol (can calculate the MW for simple compounds) 1) 200 mL of 25% SOS 2) 60 ml alkaline lysis buffer...
Most questions answered within 3 hours.
-
Which of the following pairs of ions have the same electron
configuration?
I: Br− and Se2−...
asked 1 hour ago -
The Foremost Composite Materials Company is planning a two-day
sales conference for October 19-20. The conference...
asked 2 hours ago -
3) Illustrate the observed pattern of relatedness of organisms
versus adaptations to specific conditions. This means...
asked 2 hours ago -
In winter a lake has a 0.35 m thick ice layer over 1.10 m of
water....
asked 3 hours ago -
Assuming the following has been encrypted with a Vigenere cipher
below, use the method(s) and assumptions...
asked 3 hours ago -
How would I use switch statements to write a program that will
take an input of...
asked 3 hours ago -
Imagine a reaction in which methane gas combusts at a constant
pressure of 1 atm and...
asked 3 hours ago -
Two parallel wires (each 12 m in length) are separated by a
distance of 0.065 m...
asked 3 hours ago -
Suppose there were three masses at the corner of uniform
equilateral triangle. The masses are m1...
asked 3 hours ago -
Situation: A building that is 618 m above the ground floor. How
many times would a...
asked 3 hours ago -
help me and discuss one successful and one
unsuccessful international company/busines in Indonesia.whyit
succeed and why...
asked 3 hours ago -
I- Choose the best answer
Which of the following statements about the structure and
packaging of...
asked 3 hours ago