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Draw a sequencing gel that when read gives you the following DNA sequence: 5'CTCGAAGTTTCGTAA3'

Draw a sequencing gel that when read gives you the following DNA sequence:
5'CTCGAAGTTTCGTAA3'

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Answer #1

ddCTP ddGTP ddATP ddTTP

in the Sanger sequencing, we use four PCR reactions, each PCR will have one ddNTP, ddNTP lacks 3`-OH, DNA polymerase adds new nucleotide to the 31-OH of the existing nucleic acid so if ddNTP is added PCR stops there, so in the PCR reaction with ddGTP, different sied bands can be obtained in the gel, the size of the band depends on the position of the G in the sequence.

here first nucleotide is C, so the smallest band will be in the PCR with ddCTP, and the size of the band increases as the distance from the primer increases.

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