The plant Haplopappus gracilis has a 2n of 4. At premitotic S phase of the cell cycle, a radioactive nucleotide was added. This was incorporated into the newly synthesized DNA. The cells were then washed to remove all free radioactive nucleotides.
The chromosomes at prophase and telophase of the first and second mitotic divisions after the addition of radioactive nucleotides can be shown. The presence of radioactivity is shown as grains observed after autoradiography.