Problem

The reaction catalyzed by the enzyme bovine carbonic anhydrase was studied in a stopped-...

The reaction catalyzed by the enzyme bovine carbonic anhydrase was studied in a stopped-flow apparatus at pH 7.1 and temperature 0.5°C. For an initial enzyme concentration of 2.8 × 10−9 mol dm−3, initial rates as a function of [CO2]0 are (where c° ≡ 1 mol/dm3):

103[CO2]0/c°	1.25	2.50	5.00	20.0
104r0/(c° s−1)	0.28	0.48	0.80	1.55

Find k2 and KM from a Lineweaver-Burk plot.

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Solutions For Problems in Chapter 16

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