(a) Solve the Michaelis-Menten equation (16.124) for k2[E]0 and show that (16.124) can be written as r0 = −KMr0/[S]0 + k2[E]0. (b) An Eadie-Hofstee plot graphs r0 versus r0/[S]0 and generally yields more accurate results than a Lineweaver-Burke plot. Use the data of Prob. 16.93 to prepare an Eadie-Hofstee plot and then find k2 and KM. Compare with the results of Prob. 16.93. [Many other methods exist to analyze enzyme-kinetics data. See J. G. W. Raaijmakers, Biometrics, 43, 793 (1987).]
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