Is absorbance the same for protein (bsa) curve per unit mass
Is absorbance the same for protein (bsa) curve per unit mass
Homework Answers
Answer=
The absorbance is not same for protein (bsa) curve per unit mass.
explanation- The absorbance of bsa i.e. bovine serum albumin shows at 550 nm.
As the mass per unit BSA increases,the absorbance also increases.
because the concentration of protein i.e. bsa increases.
When we plot the curve of absorbance versus concentration of BSA we get a straight line .
4, If a 0.1mM solution of a protein gave an absorbance of 1 absorbance unit. What...
4, If a 0.1mM solution of a protein gave an absorbance of 1 absorbance unit. What is the concentration of a solution of this protein that after being diluted 1000 times gave an 280nm of 0.5 Absorbance units. absorbance at + ο.0001 OmM>A
1. You have generated the following standard curve after a Bradford Assay to measure Absorbance vs...
1. You have generated the following standard curve after a Bradford Assay to measure Absorbance vs samples of known BSA concentrations. Now you need to determine the amount of protein in two mitochondria samples taken from fish, one taken from fish under normal conditions and the other taken from fish under oxidative stress. The absorbance reading for the normal fish fraction was A595=175, and the absorbance reading for the stressed fish fraction was A595= 275 Bradford Assay Standard Curve y...
Question 2 0/2 points A 0.200 M BSA solution has an absorbance of 0.100. What is...
Question 2 0/2 points A 0.200 M BSA solution has an absorbance of 0.100. What is the absorbance of a 0.250 M BSA solution under the same conditions? Report your answer to 3 decimal places. Example: X.XXX Answer: 0.265 % (0.125, 125)
The following curve presents the relationship between BSA concentration and OD at 595 or 600 nm....
The following curve presents the relationship between BSA concentration and OD at 595 or 600 nm. You isolated a recombinant protein of unknown concentration. You measured the OD value of the isolated protein and found that its OD is 0.5. Using the following BSA standard curve, determine the concentration of the isolated protein in mg/ml. BSA Standard Curve 0,9 0,8 0,7 0,6 0.5 0,4 0,3 0,2 0,1 y 0,4299x 0,0218 R" # 0,99697 0,1 0,5 1,5 2,5 mg/ml
In biochemistry, we use a 'Bradford Assay' to calculate the concentration of protein in a sample....
In biochemistry, we use a 'Bradford Assay' to calculate the concentration of protein in a sample. This method uses the same principles of the standard curve from this experiment. In a Bradford Assay, a standard curve is prepared using a known protein, typically Bovine Serum Albumin (BSA), but rather than measuring the density, we can measure a different property - its 'absorbance'. The graph below is representative of a standard curve from a Bradford Assay (with the line equation displayed...
A calibration curve was created to determine the quantity of protein in a solution. The calibration...
A calibration curve was created to determine the quantity of protein in a solution. The calibration curve has the form of a straight line with the equation A = 0.01827 +0.007 where A is the corrected absorbance of the solution and 2 is quantity of protein in the solution in units of micrograms (ug). Determine the quantity of protein in a solution that has an absorbance of 0.315. A blank solution has an absorbance of 0.078. quantity of protein: HB
1. You want to prepare a 1:50 dilution of your protein extract in a total volume...
1. You want to prepare a 1:50 dilution of your protein extract in a total volume of 1000 uL. You will need ___ uL of protein extract and ___ uL of water. 2. You are provided with a solution of BSA that is 100 ug/mL and make a 10-2 dilution? What is the resulting concentration of the DILUTED BSA? 3. Which of the following statements about enzymes is true? Select one: a. Enzymes increase the rate of a chemical reaction...
5. Below are the absorption spectra of solutions of various concentrations of BSA with a new prot...
5. Below are the absorption spectra of solutions of various concentrations of BSA with a new protein assay reagent. [BSA]-0 g/d [BSA] 4.95 x 10-3 g/dl, BSA] 9.80 x 103 g/dl, [BSA] 14.56 x 10-3 g/dl, and [BSA]-19.23 x 10-3 g/d. (a) (0.5 point) Which wavelength would be most useful in order to construct a standard curve? 2u (b) (0.5 point) Would this be a good protein assay to determine BSA concentration that is above 20.00 x 10-3 05 gd1?...
Bovine serum albumin (BSA) is a biochemically useful protein. A 0.257 gram sample of bovine serum...
Bovine serum albumin (BSA) is a biochemically useful protein. A 0.257 gram sample of bovine serum albumin is dissolved in water to make 0.146 L of solution, and the osmotic pressure of the solution at 25 °C is found to be 0.658 mbar. Calculate the molecular mass of bovine serum albumin.
They performed a BSA assay to determine the concentration of an unknown solution X. The graph...
They performed a BSA assay to determine the concentration of an unknown solution X. The graph below is the BSA standard curve. In the cuvette, you add 25 ul of the unknown solution X, 200 ul of the Bradford’s reagent and 775 ul dH2O. You obtain an absorbance of 0.58 at 595nm. Use all the information to calculate the concentration of the unknown solution X. Show work Y- 0.0457x R^2- 0.9901
4, If a 0.1mM solution of a protein gave an absorbance of 1 absorbance unit. What is the concentration of a solution of this protein that after being diluted 1000 times gave an 280nm of 0.5 Absorbance units. absorbance at + ο.0001 OmM>A
1. You have generated the following standard curve after a Bradford Assay to measure Absorbance vs samples of known BSA concentrations. Now you need to determine the amount of protein in two mitochondria samples taken from fish, one taken from fish under normal conditions and the other taken from fish under oxidative stress. The absorbance reading for the normal fish fraction was A595=175, and the absorbance reading for the stressed fish fraction was A595= 275 Bradford Assay Standard Curve y...
Question 2 0/2 points A 0.200 M BSA solution has an absorbance of 0.100. What is the absorbance of a 0.250 M BSA solution under the same conditions? Report your answer to 3 decimal places. Example: X.XXX Answer: 0.265 % (0.125, 125)
The following curve presents the relationship between BSA concentration and OD at 595 or 600 nm. You isolated a recombinant protein of unknown concentration. You measured the OD value of the isolated protein and found that its OD is 0.5. Using the following BSA standard curve, determine the concentration of the isolated protein in mg/ml. BSA Standard Curve 0,9 0,8 0,7 0,6 0.5 0,4 0,3 0,2 0,1 y 0,4299x 0,0218 R" # 0,99697 0,1 0,5 1,5 2,5 mg/ml
In biochemistry, we use a 'Bradford Assay' to calculate the concentration of protein in a sample. This method uses the same principles of the standard curve from this experiment. In a Bradford Assay, a standard curve is prepared using a known protein, typically Bovine Serum Albumin (BSA), but rather than measuring the density, we can measure a different property - its 'absorbance'. The graph below is representative of a standard curve from a Bradford Assay (with the line equation displayed...
A calibration curve was created to determine the quantity of protein in a solution. The calibration curve has the form of a straight line with the equation A = 0.01827 +0.007 where A is the corrected absorbance of the solution and 2 is quantity of protein in the solution in units of micrograms (ug). Determine the quantity of protein in a solution that has an absorbance of 0.315. A blank solution has an absorbance of 0.078. quantity of protein: HB
5. Below are the absorption spectra of solutions of various concentrations of BSA with a new protein assay reagent. [BSA]-0 g/d [BSA] 4.95 x 10-3 g/dl, BSA] 9.80 x 103 g/dl, [BSA] 14.56 x 10-3 g/dl, and [BSA]-19.23 x 10-3 g/d. (a) (0.5 point) Which wavelength would be most useful in order to construct a standard curve? 2u (b) (0.5 point) Would this be a good protein assay to determine BSA concentration that is above 20.00 x 10-3 05 gd1?...
Most questions answered within 3 hours.
-
The pH of a sample of water from a river is 5.0. A
sample of effluent from...
asked 6 minutes ago -
At the beginning of the period, the Fabricating Department
budgeted direct labor of $136,500 and equipment...
asked 34 minutes ago -
Please answer all
____ 28. Rent control is usually
justified on the grounds that it protects...
asked 33 minutes ago -
PARTS A-D HAVE BEEN ANSWERED. WAS TOLD TO REPOST. ONLY ANSWER
PARTS E and F.
A...
asked 51 minutes ago -
2) You are given the task of finding a representation for a
circle in a drawing...
asked 1 hour ago -
STUDY QUESTION: Does use of diet drug fen-phen
(fenfluramine-phentermine) cause valvular heart disease?
HINT: Valvular heart...
asked 1 hour ago -
1. An object weighing 40 N rests on a surface. The coefficient
of friction is 0.35....
asked 3 hours ago -
Investor company owns 35% of investee company voting stock and
accounts for the investment under the...
asked 4 hours ago -
The number of major faults on a randomly chosen 1 km stretch of
highway has a...
asked 4 hours ago -
Consider the competitive environment of Starbuck's, Progressive
Insurance, a manufacturing firm with low turnover, or a...
asked 5 hours ago -
3. Gains from trade
Consider two neighbouring island countries called Euphoria and
Contente. They each have...
asked 7 hours ago -
A business executive has the option to invest money in two
plans: Plan A guarantees that...
asked 9 hours ago