Yes, this clone will be useful in isolating the gene for Duchene muscular dystrophy (DMD), if translocation is the cause of the DMD mutation.
In in-situ hybridization, a certain clone is found to bind with only X chromosome, in a boy with no disease symptoms. But in a boy with Duchene muscular dystrophy, it was observed that the clone is bound to both X chromosome and to an autosome.
In in-situ hybridization, the clone hybridizes to bind with both X chromosome and an autosome. The clone spans a translocation breakpoint that involves the X chromosome and an autosome. The DMD gene binds to X chromosome normally. In this case, a translocation of the X chromosome is found, which leads to Duchene muscular dystrophy.
So, this clone can be used for isolating the gene responsible for DMD.