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Solutions For An Introduction to Genetic Analysis Chapter 14 Problem 14P

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Solution 1

This approach is not reasonable for the adjacent unique sequences. The end sequences are used as primers to link the contigs and to bridge the gap.

It is not possible to bridge a gap between adjacent unique sequences. The repetitive elements that are dispersed can cause reads from different chromosomes or different parts of the same chromosome to be aligned together wrongly.

Genome sequencing has many difficulties in eukaryotes. It is because of the existence of numerous classes of repeated sequences. Some of these repetitive sequences are arranged in tandem and others are dispersed.

If we question why these repetitive elements are a problem for genome sequencing, it is because sequencing read of repetitive DNA fits into many places in the draft of the genome. A tandem repetitive sequence is longer than the maximum length of sequence read.

So, there is no way to bridge a gap between adjacent unique sequences.

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Solutions For Problems in Chapter 14