Solutions For An Introduction to Genetic Analysis Chapter 14 Problem 4P

ESTs:

The ESTs are genome landmarks and gene discovery resources.

The ESTS are cDNA sequence reads from 5' or 3' or both ends that have been sequenced.

It is used to determine the boundaries of the 3' and 5' ends of the transcripts.

Similarly, ESTs help in the research with a quick and inexpensive route for discovering new genes, getting data about gene expression and regulation.

ESTs represent a copy of the interesting part of the chromosome and it has been a powerful tool in the hunt of genes involve in hereditary diseases.

As the ESTs can be generated quickly and inexpensively, only one sequencing experiment is needed per each cDNA produced. No error checking is needed as the errors do not prevent identification of the gene from which the EST was derived.

Thus, this helps the scientists to construct genome maps. The 3' ESTs serve as a common source of sequence tagged site (STS) because they are unique to a particular species and give additional feature of pointing directly to an expressed gene.