The sequence contigs from a genomic clone of the human species would be:
Read1: ATGCGATCTGTGAGCCGAGTCTTTA
Read2: AACAAAAATGTTGTTATTTTTATTTCAGATG
Read3: TTCAGATGCGATCTGTGAGCCGAG
Read4: TGTCTGCCATTCTTAAAAACAAAAATGT
Read5: TGTTATTTTTATTTCAGATGCGA
Read6: AACAAAAATGTTGTTATT
The reads can be written in an overlapping way:
ATGCGATCTGTGAGCCGAGTCTTTA
AACAAAAATGTTGTTATTTTTATTTCAGATG
TTCAGATGCGATCTGTGAGCCGAG
TGTCTGCCATTCTTAAAAACAAAAATGT
TGTTATTTTTATTTCAGATGCGA
AACAAAAATGTTGTTATT
a. The sequence contig of this part of H.sapiens genome is
5'TGTCTGCCATTCTTAAAAACAAAAATGTTGTTATTTTTATTTCAGATG CGATCTGTGAGCCGAGTCTTTA 3'
There are two possible transcripts as the DNA sequence can represent the template strand or the complimentary strand. The two strands are:
5'UGUCUGCCAUUCUUAAAAACAAAAAUGUUGUUAUUUUUAUUUCAGAUGCGAUCUGAGCCGAGUCUUUA-3'
and
3'ACAGACGGUAAGAAUUUUUGUUUUUACAACAAUAAAAAUAAAGUCUACGCUAGACACUCGGCUCAGAAAU5'
b. Translating the sequence contig in all possible reading frames. There are 6 reading frames. They are:
Translation of first transcript:
Frame 1starting at first letter CLPFLKTKMLLFLFQMRSVSRVF
Frame2 starting at second letter VCHS Stop
Frame 3starting at third letter SAILKNKNVVIFISDAICRPSL
Translation of second transcript:
Frame 4 starting at the first letter reads Stop
Frame5starting at the second letter reads KDSAHRSHLK stop
Frame6starting at the third letter reads KTRLTDRI stop
c. When we run the above nucleotide sequence of the contigs in BLASTn search, or if we use the translated sequence in tBLASTn search, we can find that this sequence given above is a perfect match with a specific region of the human genome. A region of the 19th exon of the CFTR gene matches correctly with the above sequence.