In corn, the allele Pr stands for green stems and pr stands for purple stems. A plant with the genotype pr/pr with standard chromosomes is crossed with a plant with the genotype Pr/Pr which is homozygous for a reciprocal translocation between chromosomes 2 and 5.
The F1 progeny is semisterile and has the phenotype of Pr. A backcross of this plant with the parent pr/pr, gives the following progeny:
• 764 – semisterile Pr
• 145 – semisterile pr
• 186 – normal Pr
• 727 – normal pr
The breakpoint can be treated as a gene with two alleles. One allele gives normal fertility while the second gives semisterility. The progeny can be grouped as shown below:
Parentals:
• 764 – semisterile Pr
• 727 – normal pr
Recombinants:
• 145 – semisterile pr
• 186 – normal Pr
The total is 1822.
The map distance between the Pr locus and the translocation point can be calculated in this way: