To create an allotetraploid between two related diploid species with the 2n number of 28, we need to do the following steps:
• Create a hybrid plant by crossing the two diploid plants
• This hybrid will also be diploid
• The chromosomes are then doubled by colchicine treatment
• This gives the allotetraploid
Another method can be as follows:
• Somatic cells are isolated from the two diploid plants
• These two different somatic cells are fused
• The fused cell will be a tetraploid
• This cell can then be grown using various culturing techniques