Two mutations ad-3 and pan-2 are present on chromosomes 1 and 6 respectively in Neurospora. An unusual ad-3 strain gives results as tabulated below:
The first cross shows independent assortment for the two genes. Since two normal strains are crossed, the ascospores are also normal in color. A recombinant frequency of 50% means that the genes are unlinked and are present on separate chromosomes.
The second cross now shows that the genes are linked, since the recombination frequency is 1%. In addition, half the progeny are inviable. This data suggests the occurrence of a reciprocal translocation. Both these genes are very close to the breakpoints of the translocation.
Abnormal ad-3 x normal pan-2:
The position of the genes:
In the third cross, we see that viable spores are of two types. Half the spores contain normal chromosomes while the other half contains translocated chromosomes.