Problem

Solutions For An Introduction to Genetic Analysis Chapter 4 Problem 45P

Step-by-Step Solution

Solution 1

A geneticist wants to obtain a corn plant with the three dominant phenotypes of anthocyanin (A), long tassels (L), and dwarf plant (D). The geneticist crosses the pure lines of AA LL dd and aa ll DD. She testcrosses the resulting F1 hybrid. The three genes are linked and the distance between A/a and L/l loci is 16 m.u. and the distance between L/l and D/d loci is 24 m.u.

a) A diagram of the chromosomes of the parents, hybrid, and tester would be as follows:

Parent AA LL dd:

Picture 1

Parent aa ll DD:

Picture 2

Hybrid:

Picture 3

Tester:

Picture 5

b) A diagram of the crossover needed to obtain the desired genotype of Aa Ll Dd would be as follows:

Picture 6

c) The map distance between these two genes includes both the single and double crossover events. The desired genotype cannot be obtained by double crossovers and hence has to be subtracted to calculate the percentage of test progeny with the desired genotype.

The expected double crossover would be the chance of recombination in one genetic interval multiplied by the chance of recombination in another interval.

The 24% recombination between genes L and D includes the 3.8% of double crossovers and 20.2% of single crossovers.

Hence, the percentage of Aa Ll Dd progeny from the testcross would be:

d) In the calculation of the frequency of the double crossovers, the assumption was made that both the crossovers were independent and there was no interference.

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