The distance between the R and S loci is 35 m.u. apart. When a plant with a genotype of RS/rs is selfed, the phenotype of the progeny and their proportions can be calculated in this way:
Parents are
Gametes | Proportion |
R S | 1/2 (1 – 0.35) = 0.326 |
r s | 1/2 (1 – 0.35) = 0.326 |
R s | 1/2 (0.35) = 0.176 |
r S | 1/2 (0.35) = 0.176 |
So, each gamete pair has enough proportion of distance.
The gametes are crossed in F1 generation, for which 10 combination of genotypes are formed. Now, calculate F1 genotypes for each combination in following manner:
Gametes | Proportion |
RS/RS | 0.325 x 0.325 = 0.1056 |
rs/rs | 0.325 x 0.325 = 0.1056 |
rs/rS | 2 x (0.325 x 0.175) = 0.1138 |
rs/Rs | 2 x (0.325 x 0.175) = 0.1138 |
RS/rS | 2 x (0.325 x 0.175) = 0.1138 |
RS/Rs | 2 x (0.325 x 0.175) = 0.1138 |
RS/rs | 2 x (0.325 x 0.325) = 0.2113 |
Rs/Rs | 0.175 x 0.175 = 0.0306 |
rS/rS | 0.175 x 0.175 = 0.0306 |
Rs/rS | 2 x (0.175 x 0.175) = 0.0613 |
Now, calculate proportion of F1 phenotypes in the following manner:
Gametes | Proportion |
RS | 0.1056 + 0.1138 + 0.1138 + 0.2113 + 0.0613 = 0.6058 |
rs | 0.1056 |
Rs | 0.1138 + 0.0306 = 0.1444 |
rS | 0.1138 + 0.0306 = 0.1444 |