Eleven different pairs of Neurospora loci of the types a b and a+ b+, and 100 linear asci were analyzed from each cross. The results are tabulated as shown:
All asci are classified as parental ditype (PD), non-parental ditype (NPD), or tetrad (T) and analyzed as to whether segregation has occurred in the first or second meiotic division. This can be done using the following table:
For cross 1: PD = NPD
The frequency of recombinants (RF) can be calculated as follows:
Hence, the genes are not linked.
For a centromere, the distance between the gene and centromere:
Hence, gene a is close to the centromere.
For b centromere:
For cross 2: PD >> NPD
The RF can be calculated as follows:
Hence, the genes are linked.
For distance between a and b –
For a centromere, the distance between the gene and centromere:
Hence, gene a is close to the centromere.
For b centromere:
For cross 3: PD >> NPD
The RF can be calculated as
Hence, the genes are linked.
For distance between a and b –
For a centromere, the distance between the gene and centromere:
Hence, gene a is close to the centromere.
For b centromere:
For cross 4: PD >> NPD
The RF can be calculated as
Hence, the genes are linked.
For distance between a and b –
For a centromere, the distance between the gene and centromere:
For b centromere:
For cross 5: PD = NPD
The RF can be calculated as
Hence, the genes are not linked.
For a centromere, the distance between the gene and centromere:
For b centromere:
For cross 6: PD >> NPD
The RF can be calculated as
Hence, the genes are linked.
For distance between a and b –
For a centromere, the distance between the gene and centromere:
For b centromere:
For cross 7: PD >> NPD
The RF can be calculated as
Hence, the genes are linked.
For distance between a and b –
For a centromere, the distance between the gene and centromere:
For b centromere:
For cross 8: PD = NPD
The RF can be calculated as
Hence, the genes are not linked.
For a centromere, the distance between the gene and centromere:
For b centromere:
For cross 9: PD >> NPD
The RF can be calculated as
Hence, the genes are linked.
For distance between a and b –
For a centromere, the distance between the gene and centromere:
For b centromere:
Cross 10: PD = NPD
The RF can be calculated as
Hence, the genes are not linked.
For a centromere, the distance between the gene and centromere:
For b centromere:
Cross 11: PD = NPD
The RF can be calculated as
Hence, the genes are not linked.
For a centromere, the distance between the gene and centromere:
For b centromere:
The map distances can be shown in the following ways: