A geneticist makes two 3-point crosses with the genes A, B, C, D, and E. The first cross of pure line genotypes are:
A/A B/B C/C D/D E/E x a/a b/b C/C d/d E/E
The F1 progeny is crossed with a recessive tester and the progeny is tabulated.
The second cross of pure line genotypes are:
A/A B/B C/C D/D E/E a/a B/B c/c D/D e/e
The F1 progeny is crossed with a recessive tester and the progeny is tabulated.
Given that the genes D and E assort independently.
a) In the first cross, since the genes C and E are same for all the progeny, the parental genotypes of the first cross can be written as:
Parents: A/A B/B D/D a/a b/b d/d
The F1 genotype: A/a B/b D/d a/a b/b d/d [tester]
The testcross progeny shows that these three genes are linked.
The distance between the genes A and B:
The distance between the genes B and D:
In the second cross, since the genes B and D are the same for all progeny, the parental genotypes can be written as:
Parents: A/A C/C E/E a/a c/c e/e
The F1 genotype: A/a C/c E/e a/a c/c e/e [tester]
The testcross progeny shows that these three genes are linked.
The distance between the genes A and C:
The distance between C and E:
The map showing the gene distances:
b) Interference can be calculated using the following formula:
For cross 1:
Therefore, cross 1 shows no interference.
For cross 2:
Therefore, cross 2 shows no interference.