Three different crosses of the organism Neurospora are analyzed on the basis of unordered tetrads seen. The results of the cross are tabulated:
a) The frequency of recombinants can be calculated using the following formula:
Where, NPD stands for non-parental ditypes
T stands for tetrad.
For cross 1:
For cross 2:
For cross 3:
b) The uncorrected map distance based on RF can be calculated using the formula:
Where, NPD stands for non-parental ditypes
T stands for tetrad.
Total is the sum of PD, NPD, and T.
For cross 1:
For cross 2:
For cross 3:
The uncorrected map distances are thus calculated.
c) The corrected map distances based on tetrad frequencies can be calculated using the formula:
Where, NPD stands for non-parental ditypes
T stands for tetrad.
Total is the sum of PD, NPD, and T.
For cross 1:
For cross 2:
For cross 3:
d) The corrected map distance based on the mapping function:
Where, e = the base of natural logarithms
m = the mean number of successes in a defined sample size.
For cross 1:
For cross 2:
For cross 3: