The dihybrid cross of two parents E/E F/F and e/e f/f were crossed to get F1 progeny. The F1 parent is backcrossed to the recessive parent with the genotype e/e; f/f.
There are four distinct genotypes resulted from the above cross:
EeFf: 25%
Eeff: 25%
eeFf: 25%
eeff: 25%
The phenotypic ratio for the same cross is 1:1:1:1.
In the given progeny, the genotypes are in the following proportions:
• EF – 2/6
• Ef – 1/6
• eF – 1/6
• ef – 2/6
If independent assortment of genes occurs, the ratio of any organism with a homozygous recessive organism would yield the ratio of 1:1:1:1, which is not observed in given question. This is due to linked genes.
The recombinants are eF and Ef and the number of recombinants is 2/6 or 1/3.
Hence, the distance between the two genes would be calculated as:
So, the two genes are 33.3 mu apart.