a) The given sequence of double stranded DNA molecule is as follows:
5' - TACATGATCATTTCACGGAATTTCTAGCATGTA - 3'
3' - ATGTACTAGTAAAGTGCCTTAAAGATCGTACAT- 5'
The 5' - TACATGATCATTTCACGGAATTTCTAGCATGTA 3' is primarily transcribed after the RNA polymerase identifies TCA sequence in 3'- TAC - 5' direction in DNA.
b) The 5' and 3' ends of the given DNA strand is as follows:
5' - TACATGATCATTTCACGGAATTTCTAGCATGTA - 3' - Sense strand
3' - ATGTACTAGTAAAGTGCCTTAAAGATCGTACAT- 5' - Antisense strand
The upper strand of the given DNA molecule possesses the required codons.
DNA: 3' TAC GAT CTT TAA GGC ACT 5'
RNA: 5' AUG CUA GAA AUU CCG UGA 3'
Protein: Met Leu Glu Ile Pro Stop
c) By considering the polarity, the inversion is
DNA: 5' TAC GTA ATC GAA ATT CCG TGA AAT GAT CAT GTA 3'
RNA: 3' -GAU CUU UAA GGC ACU UUA CUA GUA- 5'
Amino acids: HOOC- 7 6 5 4 3 2 1 - NH2
d) DNA: 3' ATG TAC TAG TAA AGT GCC TTA AAG ATC GTA CAT 5'
mRNA 5' UAC AUG AUC AUU UCA CGG AAU UUC UAG 3'
1 2 3 4 5 6 7 Stop
5' -UCA- 3' codon which is in 4th place codes for Serine. The anticodon is 3' - AUG- 5'.