The transcription is a process in which a particular segment of deoxyribonucleic acid is imitated in to ribonucleic acid. This process is done by using an enzyme called RNA polymerase. During the process of transcription, the deoxyribonucleic acid sequence is read by the enzyme RNA polymerase. This enzyme produces a ribonucleic acid strand which is complementary and antiparallel to deoxyribonucleic acid.
a) By using the top strand of DNA segment 5' GCTTCCCAA 3' as a template by the RNA polymerase the RNA transcribed is as follows:
UUGGGAAGC
b) The transcribed RNA is labelled as 5' UUGGGAAGC 3'. The transcription is a process in which a particular segment of deoxyribonucleic acid is imitated in to ribonucleic acid. This process is done by using an enzyme called RNA polymerase. During the process of transcription, the deoxyribonucleic acid sequence is read by the enzyme RNA polymerase. This enzyme produces a ribonucleic acid strand which is complementary and antiparallel to deoxyribonucleic acid.
c) The transcription is a process in which a particular segment of deoxyribonucleic acid is imitated in to ribonucleic acid. This process is done by using an enzyme called RNA polymerase. During the process of transcription, the deoxyribonucleic acid sequence is interpreted by the enzyme RNA polymerase. This enzyme produces a ribonucleic acid strand which is complementary and antiparallel to deoxyribonucleic acid.
The amino acid chain for the transcribed RNA is as follows:
UUG - Leu
GGA - Gly
AGC – Ser
d) The amino acid chain has to be labeled with its amino and carboxyl ends.
NH3 – Leu – Gly – Ser – COOH
By using the bottom strand 3' CGAAGGGTT 5' as template:
• The RNA transcribed and labeled is 5' CGUUCCCAA 3'.
• The amino acid chain with its ends for the transcribed RNA will be as follows:
NH3 – Ala – Ser – Gln - COOH